Stage One: Inject the bag
The first stage involves growing the white mushroom mycelium on a bag of sterile rye grain.
We use rye as it is easy to mix and allows the mushroom to spread quickly through it.
This method is suitable for many different species of mushroom. Temperatures may vary depending on the species grown.

Wipe injector site and needle with
swab
Inject 2-5 mL of
edible mushroom culture. You can store the leftover syringe in the fridge. (N.B. Injecting more liquid won't do any harm but doesn't speed things up either).
Stand the plastic up vertically. Incubate at 25-30 C in the dark (airing cupboards are good).
White mycelia grows through bag. After 7-10 days you can speed things up by gently mixing the bags contents (Do not open the bag! Gently break up the grain and mix through the plastic with your hands). It should be fully white (or "colonised") within about 20-30 days.
Stages of growth. The bag on the right is fully colonised (i.e. completely white). When the bag is completely white move on to
Stage Two: Casing